HCS Reagent Selection Guides

High-content analysis reagents

Partner with us to take advantage of the expertise and reliability of our instruments, technology, as well as decades of experience in labeling and detection with Invitrogen Molecular Probes fluorescence reagents.

Explore how employing decades of experience in fluorescence imaging and Thermo Scientific high-content platforms develops complementary Invitrogen HCS reagents with special considerations for the high-throughput workflow and automated imaging:

Cell and nuclear masks for automated demarcation
Robust functional probes for cell health interrogation
Flexible assay workflow for automated processing
Broad fluorophore choice for easy multiplexing
Validated on multiple imaging platforms

Select from the reagents optimized for use with your HCS platform:

CellInsight CX5 reagents

BPAE cells imaged using the CellInsight CX5

BPAE cells imaged using the Thermo Scientific CellInsight CX5 platform and stained with DAPI (blue), Invitrogen Alexa Fluor 568 phalloidin (red), goat anti–mouse IgG (H+L) secondary antibody, and Alexa Fluor 488 conjugate (green).

CellInsight CX7 reagents

A549 cells imaged using the CellInsight CX7 platform

A549 cell image acquired using the CellInsight CX7 platform and stained with Invitrogen HCS CellMask Blue (blue), Alexa Fluor 488 Phalloidin (green), and Alexa Fluor 750 goat anti–mouse IgG secondary antibody (pink).

CellInsight CX7 LZR reagents

Fibroblasts were stained with Hoechst 33342 (blue), Histone H3 (white), and Phalloidin (red) then imaged using confocal imaging with CellInsight CX7 LZR and 70 micron confocal pinhole diameter.

Other cell structure and segmentation tools

Clear identification of cell and nuclear boundaries in automated imaging requires fiducial markers that will label cellular structures robustly and reproducibly. Cell masks are designed specifically for automated imaging. Invitrogen Molecular Probes cell structure labels are widely used in microscope imaging and HCS.

See reagents for cell structure

Other cell health and cytotoxicity assays

We offer a diverse selection of imaging and microscopy assays for the analysis of cell viability, proliferation apoptosis, cell cycle and DNA repair, migration, adhesion and other cellular functions. Many of these assays have been validated specifically for HCS platforms.