Alexa Fluor 532 dye

Alexa Fluor® 532 dye Alexa Fluor 532 is a yellow-fluorescent dye with excitation ideally suited for the frequency-doubled Nd:YAG laser line. Alexa Fluor 532 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.

Alexa Fluor 532 conjugates exhibit an excitation maximum at 532 nm and emission maximum at 554 nm. It is particularly useful in multicolor applications, including super-resolution microscopy using dSTORM.

We offer Alexa Fluor 532 dye conjugated to a variety of antibodies and peptides optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to enable you to generate your own antibody conjugates or probes.

Alexa Fluor 532 dye dashboard

Initial brightness		Alexa Fluor 532 dye is used in stochastic optical reconstruction microscopy (STORM)—as an activator in nSTORM and a reporter in dSTORM.

Photostability in buffer

Photostability in antifade

Performance in SRM

	532	TRITC	532	554
	Laser line	Common filter set	Excitation max	Emission max

Alexa Fluor 532 dye products

We offer a broad range of Alexa Fluor 532 products including protein and antibody labeling kits, secondary antibodies, and bioconjugates:

See all Alexa Fluor 532 Primary Antibodies
See all Alexa Fluor 532 Secondary Antibodies

Human mononuclear cells were stained with mouse anti human CD4 biotin, washed, stained with streptavidin, Alexa Fluor 532 conjugate, washed, and analyzed by flow cytometry using 532 nm excitation.

Alexa Fluor 532 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups:

Amine	Thiol
NHS ester	Maleimide

Alexa Fluor 532 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target protein	Kit	Cat. No.
Site-specific antibody labeling
<1–20 µg mouse IgG1	Zenon Alexa Fluor 532 Mouse IgG1 Labeling Kit	Z25003
<1–20 µg rabbit IgG	Zenon Alexa Fluor 532 Rabbit IgG Labeling Kit	Z25303
Conventional antibody/protein labeling
100 µg antibody/protein	Alexa Fluor 532 Antibody/Protein Labeling Kit	A20182
1 mg antibody/protein	Alexa Fluor 532 Antibody/Protein Labeling Kit	A10236