APC-eFluor 780 Dye

graph of emission spectra for APC-eFluor 780 dye

APC-eFluor 780 organic dye was developed to provide a high performance tandem fluorophore for multicolor flow cytometry. It was designed for use with flow cytometry instruments equipped with a red laser and has an emission peak of 780 nm. With similar spectral characteristics to APC-Alexa Fluor 750, APC-eFluor 780 requires no changes to your instrument configuration for use, allowing you to easily add APC-eFluor 780 to your multicolor panels.

Benefits of APC-eFluor 780 include

Equal or better sensitivity than APC-H7 tandem conjugates
Stable performance when treated with formaldehyde fixation or exposed to ambient light
Uses same filter sets as existing comparative fluorochromes

APC-eFluor 780 dye dashboard

Initial brightness		APC-eFluor 780 dye has similar spectral properties to APC-H7, APC-Cyanine7 and APC-Alexa Fluor 750 dye. It can replace any of these tandem dyes when designing a multicolor staining panel. The APC-eFluor 780 tandem dye is easily compensated when used with APC.

Photostability in buffer

	633	780/60	655	780
	Laser line	Common filter set	Excitation max	Emission max

Flow histogram comparing APC-eFluor 780 dye to APC-H7

Comparison with APC-H7. Mouse splenocytes stained with Anti-CD8 (clone 53-6.7) conjugated to APC-eFluor 780 (blue line) or APC-H7 (red line).

Flow histogram comparing APC-eFluor dye 780 before and after aldehyde fixation

Stability of APC-eFluor 780. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-CD8 APC-eFluor 780 (clone RPA-T8) and analyzed immediately (red line). The sample was then divided and either exposed to ambient light for six hours (blue line) or fixed in 2% formaldehyde for 30 minutes at room temperature (green line) and analyzed again.