PCR Learning Center

Close-up view of a DNA helix with more helices in the background

Learn about PCR methods and technologies

The polymerase chain reaction, or PCR, is one of the key techniques in molecular biology, amplifying a single DNA molecule into millions of copies in a short time by a biochemical process. It has a wide range of applications from basic research to disease diagnostics, agrigenomics, and forensics.

This PCR Learning Center provides entry points to educational materials, which are designed to empower scientists and researchers like you with PCR knowledge, to help you succeed in your experiments regardless of your level of expertise. Select a topic below to view a collection of learning resources to fit your needs—from articles, application notes, videos, webinars, and more.

PCR subtopics for learning

PCR enzymes and reagents

DNA polymerase with engineering schematics

Review the history and basis of PCR, considerations for reaction setup, properties of DNA polymerases, common PCR methods and applications, and troubleshooting PCR experiments.

PCR primers

Get answers on frequently asked questions about DNA oligos for PCR, such as the maximum length for synthesis, determination of full-length oligos, and reconstitution of received oligos.

PCR plastics and seals

PCR plate with schematics of frame and 96 wells

Learn about essentials of PCR/qPCR plastics and seals, their impact on PCR and data collection, and tips on troubleshooting common issues of PCR/qPCR plastics consumables.

Thermal cyclers

Thermal cycler block with 6 temperature zones

Review innovation of thermal cyclers since their introduction in the 1980s, technological advances that enable PCR improvement, and important considerations for thermal cyclers in PCR success.

Reverse transcription (RT)

Review the basics of reverse transcription, considerations for reaction setup, considerations for choosing reverse transcriptases, common applications of reverse transcription, and troubleshooting for cDNA synthesis.